Proteins are the direct performers of life functions. The quantitative proteomics service is based on the high-resolution electrospray Orbitrap and 4D-timsTOF mass spectrometry platform, providing precise quantification of complex proteomes in samples. It is particularly suitable for biomarker screening, drug concept verification and signal network analysis.
1. DIA (data independent acquisition) omics: It has excellent measurement reproducibility, no missing values, and is suitable for large-scale sample analysis.
2. TMT (tandem mass spectrometry labeling) / iTRAQ quantification: Supports simultaneous labeling and same-needle mass spectrometry detection of up to 16 parallel samples.
3. Modified proteomes (PTMs): Provides a specific antibody enrichment platform, including quantification of phosphorylation, acetylation, ubiquitination and glycosylation modifications.
Quantitative proteome analysis delivers highly reproducible expression matrices, volcano plots, cluster heat maps, and functionally enriched pathway maps.
| Technical indicators | Service Parameters and Delivery |
|---|---|
| Main instruments | High-resolution Orbitrap mass spectrometer / 4D timsTOF mass spectrometer |
| Detection depth | A single DIA needle can stably compare 8000+ protein types |
| Minimum database construction | A minimal protein purification system was developed for clinical needle aspiration biopsy tissue. |
| In-depth analysis of bioinformation | Volcano plot, pathway clustering heat map, protein interaction network (PPI), multi-omics association pathway enrichment |
Protein extraction and pre-processing have high requirements on sample quality. Please ensure that the following parameters are met when sending samples:
| Sample type | Quantity requirements | Extraction/Pre-processing Recommendations | Transportation form |
|---|---|---|---|
| Fresh animal and plant tissues | Animal tissue > 50mg, plant tissue > 100mg | Liquid nitrogen quick freezing, avoid repeated freezing and thawing | Ultra-low temperature dry ice sealed transportation |
| cell pellet | ≥ 1 × 10^7 cells | Remove the culture medium, wash with PBS, centrifuge to remove the supernatant, and freeze in liquid nitrogen | Ultra-low temperature dry ice sealed transportation |
| Serum/Plasma | ≥ 200 µL/sample | Centrifuge to remove red blood cells to avoid hemolysis | Ultra-low temperature dry ice sealed transportation |
| Puncture tissue (micro volume) | 3-5 puncture needle cores | Quickly freeze in ultra-low temperature freezing tube | Ultra-low temperature dry ice sealed transportation |
Metabolites, as the end products of physiological reactions, can more sensitively and directly map the perturbations of phenotypes caused by external environmental stimuli or mutations in the body. HST GENOMICS combines advanced liquid chromatography mass spectrometry (LC-MS) technology to provide systematic analysis covering hundreds to thousands of metabolites.
1. Untargeted metabolomics (Untargeted): Unbiased acquisition of the full spectrum of metabolite changes in samples, most suitable for biological discovery and early diagnostic marker establishment.
2. Highly sensitive targeted quantification: Accurate absolute quantification of standard calibration for core pathway small molecules such as bile acids, amino acids, short-chain fatty acids, etc.
3. Lipidomics and metabolic flux: Deep coverage of polar/non-polar molecules such as triglycerides and phospholipids, and supports isotope tracer analysis.
Metabolome analysis delivers qualitative and quantitative score tables, multivariate statistical charts (PCA, OPLS-DA), VIP importance maps and pathway enrichment maps.
| analysis project | Deliver diagrams/models | Main technical uses |
|---|---|---|
| Qualitative identification of substances | Secondary fragment comparison score table, high-resolution accurate molecular weight | Accurately identify endogenous and exogenous small molecule metabolites |
| multivariate statistical analysis | PCA scatter plot, PLS-DA/OPLS-DA discriminant analysis | Evaluate overall sample differences and separation trends between groups |
| Marker screening | VIP value percentage chart, volcano chart | Target the significantly different metabolites that contribute most to classification between groups |
| pathway mapping | KEGG metabolic pathway bubble diagram, pathway association network | Regulatory network connecting differential small molecules and upstream genes/proteins |
Metabolites have weak stability and are susceptible to external contamination. Please strictly abide by the following sample delivery standards:
| Sample type | Quantity requirements | Extraction/Pre-processing Recommendations | Transportation form |
|---|---|---|---|
| plasma/serum | ≥ 100 µL/sample | Immediately after collection, centrifuge to remove cells to avoid hemolysis and quick freeze | Ultra-low temperature dry ice sealed transportation |
| Urine/cerebrospinal fluid | ≥ 200 µL/sample | Centrifuge to remove cell debris and freeze quickly | Ultra-low temperature dry ice sealed transportation |
| animal and plant tissue | ≥ 100 mg | Quickly peeled on ice, washed with PBS, and quick-frozen in liquid nitrogen | Ultra-low temperature dry ice sealed transportation |
| feces/intestinal contents | ≥ 100 mg | Collect in sterile cryovials and quickly put into liquid nitrogen or -80℃ | Ultra-low temperature dry ice sealed transportation |