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Helix-SC Microfluidics 500 - 80,000 cell throughput >65% capture specificity CITE-Seq protein detection

System overview

Helix-SC single cell systemIt is the core hardware to solve the problem of organizational heterogeneity. Based on high-precision droplet microfluidic partitioning technology, the device distributes suspended cells into upgraded independent water-in-oil droplet microstructures (GEMs) at high speed, enabling concurrent barcode labeling and library capture of tens of thousands of cells.

Core advantages

Traditional whole-tissue sequencing can mask critical transition states and minor subpopulations due to signal averaging. Helix-SC provides a clear representation of cellular diversity within a sample and can be used to identify rare subpopulations and cell differentiation slopes within the tumor immune infiltrate. The device deeply supports CITE-Seq, which can simultaneously analyze transcriptome and surface membrane protein phenotypes in one shot.

Technical indicators design parameters
running flux Supports capture of 500 - 80,000 viable single cells in a single run
capture efficiency High standard cell retention rate >65%, reducing the loss of precious samples
System process 1. Microfluidic droplet coating; 2. Cell lysis and barcode synthesis; 3. Library PCR amplification; 4. HST-Loupe cluster analysis
Multi-omics compatible Single cell transcript expression (3' scRNA), single cell chromatin openness (scATAC)
Cell IF: 45.5 Application platform: Helix-SC single cell system
Single-cell transcriptome profiling reveals the evolution of immune exhaustion of heterogeneous T cells in the tumor immune microenvironment
Research background: Immune checkpoint blockade therapy is only effective in some tumor patients, requiring high-resolution analysis of the heterogeneous functional status of tumor-infiltrating immune cells.
research methods: The Helix-SC single-cell system was used to capture 100,000 single cells from tumor tissues, adjacent cancer cells, and peripheral blood of 20 liver cancer patients for high-fidelity single-cell transcriptome sequencing.
Main conclusions: Mapping a detailed T cell exhaustion differentiation trajectory and identifying a group of rare CD8+ T cell subpopulations that specifically express exhaustion markers, providing important targets for the development of new immune combination treatments.

Ordering and Configuration Instructions

1. Standard single cell workstation: Contains Helix-SC controller host, high-precision microfluidic sampling pump and standard data flow analysis workstation. A multi-omics research platform suitable for all types of universities and scientific research institutes.

2. Multi-omics upgrade package: Based on the standard version, it provides scATAC-seq and CITE-Seq (surface protein simultaneous detection) upgraded accessories and adapter tips, which are suitable for conducting in-depth epigenomic and single-cell proteome research.

3. Joint ordering of reagents and consumables: Helix-SC is equipped with single cell sorting chips and library construction kits. It is recommended to package and subscribe to ensure the consistency of consumable batches, and enjoy a full set of Chinese experimental SOP guidance and technical training.

For an equipment quote, trial request, or sample preparation guide, visit Contact us Submit your application.

Spatial-Map in situ 55 μm resolution Whole transcriptome capture FFPE / Frozen Section

System overview

Spatial structure is a core element in understanding organizational microenvironments.Spatial-Map spatial tissue imagerPerfectly combine high-definition cell tissue morphology with ultra-high-throughput whole-transcriptome sequencing. There is no need to mechanically dissociate the tissue into a suspension through enzymatic methods, and gene transcription information can be obtained directly while retaining the physical spatial topology.

Observe and regulate in situ

This device captures mRNA released in situ from slices through a patented spatial glass slide chip (integrated with a 55 μm diameter specific barcode probe array). This allows you to visually render gene expression levels on H&E or immunofluorescence images, and clearly decipher the invasion heterogeneity at the tumor edge and the molecular partitioning of the brain's neural network.

Technical parameters Design specifications
spatial resolution 55 μm probe array diameter enables high-precision positioning at the cell community level
Chip capture area 6.5 mm x 6.5 mm or 11 mm x 11 mm optional
Sample compatibility Maturity supports Fresh Frozen and Formalin Paraffin Embedded Section (FFPE) samples
capture content Total transcriptome (Total mRNA) without specific probe restrictions
Supporting software HST-Loupe spatial three-dimensional reconstruction and image superposition comparison system
Nature IF: 50.5 Application platform: Spatial-Map spatial tissue imaging system
Spatial transcriptome technology maps fine spatial structure and gene expression map of mammalian cerebral cortex
Research background: The brain has extremely complex physical partitions and functional anatomy. Traditional in vitro single-cell analysis will lose the physical topological connections between neurons.
research methods: The Spatial-Map spatial tissue imager was used to hybridize and capture mouse brain sections, and while retaining the morphological characteristics of H&E-stained brain regions, the whole transcriptome gene expression was measured.
Main conclusions: Successfully mapped the fine spatial gradient distribution of different cell levels in the cerebral cortex, and discovered several spatial molecular markers that specifically control neuron positioning and synaptic connections.

Spatiomics Hardware and Chip Selection Guide

1. Space in-situ scientific research configuration: Contains Spatial-Map imager host, special slide transillumination fixture, and HST-Loupe spatial visualization system. Suitable for key laboratories in the fields of tumor microenvironment and developmental biology.

2. Frozen and paraffin dual-mode chip package: Provides a spatially positioned slide and reverse transcription reagent set optimized for fresh frozen sections (FF) or paraffin sections (FFPE).

3. Spatial data workstation: Since the amount of data after superposition of spatial image data and high-throughput sequencing data is extremely large, it is recommended to select a GPU image acceleration workstation specially optimized for HST-Loupe.

To obtain slide samples, live sectioning demonstrations, or learn about academic group purchasing policies, please visit our message board Leave a message and our spaceomics experts will arrange it for you.

Supporting kit DNB sequencing library construction Single cell sorting chip spatially positioned slides

Molecular Biology Kit Matrix

In order to ensure the reproducibility and precise quality control of off-machine data, HST GENOMICS provides a complete matrix of wet experiment kits suitable for autonomous sequencing and single-cell systems.

Core product pedigree

1. Single cell capture chips and reagents: Contains Helix-SC pipetting chip, Gel Beads with cell-specific barcodes and nucleic acid amplification reagents.

2. Spatial-Map space chip box: Contains dedicated microwell slides and biochemical reagents for section in situ hybridization, permeabilization and reverse transcription.

3. High-fidelity DNB library construction reagents: Provide rolling circle synthesis cyclization reagents and high-efficiency sequencing primers without PCR amplification errors, effectively reducing the duplication rate in sequencing.

Kit name Adaptation platform Core technology and output
Single cell capture chips and reagents Helix-SC Pipetting chips, specific barcode gel beads, nucleic acid amplification reagents, cell retention rate >65%
Spatial-Map space chip box Spatial-Map In situ hybridization, permeabilization and reverse transcription slides and biochemical reagents, 55μm array capture
High-fidelity DNB library construction reagents T-Series / G-Series Rolling circle cyclization reagents and high-efficiency sequencing primers, cloning duplication rate (duplication rate) <3%
BioTechniques IF: 2.5 Application platform: DNB library construction kit
Evaluation of the improved accuracy of genomic variant identification using high-fidelity DNB library construction kits
Research background: Traditional PCR amplification library construction easily introduces amplification bias in GC- or AT-rich regions and increases the repeat data ratio.
Comparison method: Use HST high-fidelity DNB library construction reagent (free of PCR cyclization) and standard adapters to construct the library, and perform whole-genome sequencing in parallel.
Main conclusions: The sample duplication rate using DNB library construction reagent was reduced to 1.8% (8.5% in the control group), the alignment depth uniformity in the GC preference region was improved by more than 30%, and the detection accuracy of rare variants (low-frequency variants) was significantly improved.

Ordering and selection suggestions

1. Single cell flow channel kit: Single-cell sorting chips (Chip) and gel beads (Gel Beads) are available in 4-, 8- and 16-run specifications. Please purchase them appropriately based on the number of samples in your batch and store them properly at -80°C.

2. Spatial positioning of slides: Spatial-Map microarray slides are available in boxes of 2 or 4. Reagents should be used within 3 months after opening to ensure reverse transcriptase activity.

3. Bulk Subscription Agreement: For customers who have long-term large-scale projects, HST provides annual consumable price locks and batch reservation agreements to avoid batch effects during the experiment process.

All test kits have passed strict verification on independent platforms before leaving the factory. For detailed specifications and quotations, please go to Contact us Submit information.