HST-Prep Automated Liquid Handling WorkstationIt is a precision automated workbench specially designed for high-throughput sequencing (NGS) library construction, target capture, and various molecular biology separation experiments. By integrating a high-precision syringe pump and a multi-degree-of-freedom pipetting robot, the system achieves high-precision distribution and transfer of trace amounts of liquids from 1 µL to 1000 µL, completely replacing traditional tedious and error-prone manual pipetting operations.
HST-Prep has a flexible SBS layout with multiple plate positions. It integrates a Peltier temperature control module, a microplate shaker, a magnetic separation rack and a built-in PCR thermal cycler (On-deck Thermocycler) on the board to support the continuous and automatic library construction process of "sample in, library out". The equipment shell adopts a closed safety physical shield, and is equipped with a high-efficiency HEPA air laminar flow system and an automatic UV sterilization module to effectively prevent aerosol and microbial contamination and ensure the high purity and stability of clinical and R&D libraries.
| Technical indicators | Technical parameters |
|---|---|
| Pipetting accuracy (CV) | CV ≤ 5.0% at 1 µL; CV ≤ 2.0% at 10 µL; CV ≤ 1.0% at 100 µL |
| Pipetting channel | Can be configured with 1/8 independent channel or 96-channel pipetting head, supporting liquid level detection and wall-mounted residual sensing |
| Standard SBS board position | 16 to 24 plate positions (accommodates deep well plates, tip boxes, reagent tanks and temperature control kits) |
| Onboard circulation system | Integrated PCR thermal cycler with automatic opening and closing thermal lid, supports amplification of various commercial library construction kits |
| Temperature control range | Precision Peltier temperature control from 4°C to 99°C ensures optimal enzyme reaction and magnetic bead adsorption |
1. Flexible scientific research type (8 channels): Equipped with a 1/8-channel variable pitch pipetting head, it is suitable for laboratories with small sample volumes but frequent changes in experimental plans. It supports flexible dispensing in small-volume tubes and standard 96-well plates.
2. Ultra-high-throughput production type (96 channels): Equipped with 96 fixed-channel pipetting heads, on-board integrated PCR thermal cycler and 24 plate positions, it is especially suitable for large diagnostic centers and cohort projects with a daily testing volume of more than 100+.
3. Customized configuration for clinical delivery: Pre-passed GMP level factory verification, integrated HEPA safety dust cover and code scanning verification system. Includes 21 CFR Part 11 compliance audit trail activation key for software.
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LIMS intelligent control systemIt is the central brain of high-throughput molecular laboratory operations. This system breaks the "equipment island" and realizes the full life cycle traceability and intelligent logistics scheduling from receiving original sample tubes, nucleic acid extraction, concentration quality control (QC), automated library construction, to the final loading of sequencing chips on the computer.
Before each automated library construction run, the onboard 2D code scanning module performs real-time inventory verification of sample tubes and reagent barcodes, and the system automatically locks the correspondence between sample numbers, consumable batch numbers, and experimental plans (SOPs). Data such as temperature logs, tip consumption, and quality inspection readings generated during operation are automatically generated into electronic experimental reports, which are synchronized to the LIMS database in real time via API, supporting multi-person online review, and fully complying with the strict review standards of GMP and CAP/CLIA laboratories on the anti-tampering of original records.
| system module | Function description |
|---|---|
| Sample registration and tracking | Supports batch reading and verification of barcodes/2D cryotubes, and automatically generates global unique UID and circulation topology |
| Workstation Interconnect API | Seamless linkage with HST-Prep, T-Series and other devices to deliver SOP parameters in real time and recover operation logs |
| Electronic experiment report | Automatically integrate extraction concentration, QC readings and sequencing quality control, with multi-person online multi-level approval electronic signatures |
| Compliance and Audit | 100% lab operation audit trails and logs are tamper-proof and fully compliant with GMP and CAP/CLIA requirements |
| Deployment form | Local server deployment (On-Premises) or private cloud deployment (Private Cloud) |
1. Stand-alone basic version (HST-Prep companion): Contains only the interface of the liquid processing workstation and the stand-alone sample import module, suitable for small and medium-sized R&D teams equipped with 1-2 automated workstations.
2. Laboratory standard version (multi-device interconnection): Supports global networking and LIMS data center of less than 10 sequencers, quality inspection instruments and pipetting workstations. Includes complete warehouse inventory and standard QC modules.
3. Enterprise customized version (On-Premise proprietary deployment): Provide one-to-one software demand docking for large third-party inspection institutes and pharmaceutical company R&D centers. Supports login and proprietary API bridging with customers' existing ERP domain accounts (LDAP/AD).
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